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BIODETECTION TECHNOLOGIES 2014 - 바이오 탐색 기술 컨퍼런스 2014 -
2014년 6월 10 - 11일, 미국 메릴랜드주 볼티모어

         
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이번에 22회째를 맞이하는 BIODETECTION TECHNOLOGIES 2014는 Detection Technologies 시리즈 컨퍼런스로, 생물학적인 위협의 탐색과 식별 및 point-of-care 분석 방법 전문가가 한자리에 모이는 이벤트로 국제적으로도 높은 평가를 받고 있습니다. 컨퍼런스에서는 다채로운 세션이 예정되어 있으며, 생물학적 방위 기술과 생물 의학 기술의 최종사용자로부터 얻은 피드백 분석, 이 분야의 최신 연구개발 및 상업화 동향이 소개됩니다.

 
•    고속 바이오센싱, 바이오 탐색 및 분석
•    다수의 위협과 병원체 동시 탐색
•    숙주 반응 탐색을 위한 생물학적 흔적(biosignature) 발견 및 분석
•    숙주 반응 탐색을 위한 생물학적 흔적(biosignature) 발견 및 분석
•    병원체/바이러스/위협 탐색과 식별을 위한 Point-of-care/임상 응용 기술
•    자원이 한정된 검사실과 임상 현장, 모바일랩을 위한 Point-of-care 어세이
•    마이크로어레이와 시퀀싱 기술의 진보
•    현장에서 도입 가능한 기기:휴대성/호환성/신뢰성/확장성
•    비PCR 및 PCR 기반 탐색 기술
•    탐색 감도/검사/평가/검증 문제
•    동물과 식물 병원체
•    바이오일렉트로닉스
•    DNA 칩, 핵산 센서, 압타센서
•    효소 기반 바이오센서
•    면역 센서
•    랩온어칩(Lab-on-a-chip)
•    미세유체공학과 고정화 기술
•    천연 및 합성 수용체(MIP 포함)
•    유기체 및 전세포 기반 바이오센서
•    프로테오믹스, 단일 세포 분석, 암세포 탐색
•    신호 전달 기술(자기, 압전, 광학, 직접전기화학)
 
 
 
 
 
 
 
미디어 스폰서와 컨퍼런스 파트너
 
 
 
 
 
2014년 6월 10일(화)
 
8:00        등록, 전시회 견학/포스터 셋업, 커피와 다과
 
생물학적 표적 식별을 위한 유전자 증폭
 
9:00       병원체 식별 강화와 오픈어레이 플랫폼을 이용한 헌혈자 시료 멀티플렉스 검사
 
Robert Duncan, PhD, Staff Scientist, FDA Center for Biologics Evaluation and Research, U.S. Food and Drug Administration
 
Detection of pathogens in blood is required for donor screening and diagnostics. We recently demonstrated effective multiplex screening for 9 pathogens simultaneously with the OpenArray nanofluidic real-time PCR platform. The blood borne pathogen OpenArray platform has been expanded to screen 26 pathogens with discrimination to the species, strain or genotype level. High sensitivity of detection was demonstrated with 92 blood donor specimens.
 
9:30        치료 지침이 되는 버크홀데리아(Burkholderia) 검출
 
R. Paul Schaudies, PhD, CEO, GenArraytion, Inc.
 
In collaboration with USAMRIID, thirty isolates each of Burkholderia pseudomallei and mallei were screened using a genotyping microarray. Results were combined with antibiotic sensitivities to generate multiplexed real-time PCR to identify and characterize these organisms.
 
10:00    전도성 DNA에 의한 복수 타겟의 실시간 동시 탐색
 
Fred Albert, PhD, President, Bridger Technologies, Inc.
 
While progress is being made on the rapid and simultaneous detection of multiple targets, evolutionary improvements to conventional biodetection systems are unlikely to overcome their inherent limitations. A breakthrough technology that enables the simultaneous detection of multiple targets in less than one minute will be presented. This non-PCR, conductive DNA-based technology has been shown to be highly sensitive, highly specific, and deliver accurate results even in the presence of background and contaminants that debilitate or foul other detection systems.
 
10:30    휴식시간, 전시회/포스터 발표 관람
 
11:00    환경 조사를 위한 완전 자율형 바이오 탐색 시스템
 
Mark Burton, Northrop Grumman Corporation
 
The Next Generation Automated Detection System (NG-ADS) Biodetector that continuously collects and analyzes air samples to detect and identify biological threat agents will be presented.  The biodetector operates autonomously between routine consumables replenishment, and can be operated 24/7 year round. The detector is designed to use a multiplexed PCR assay to reliably detect threat agents simultaneously with high sensitivity, low limit of detection, and an extremely low false positive rate.  Samples are archived for further analysis if desired.  These systems have recently participated in a field test to demonstrate performance in an operational environment.
 
11:30    새로운 진단 전략 기반 임상상 어려운 문제에 대응
 
Harshini Mukundan, PhD, Research Scientist, Los Alamos National Laboratory
 
We will discuss advanced and integrated biodiagnostic strategies at LANL, spanning from rapid biomarker detection to advanced sequencing based analysis, to understand the circulation, emergence and occurrence of drug resistance in a pediatric population in rural Kenya. In addition, we will also talk about novel detection strategies used for the detection of bacterimia, including active tuberculosis, for the first time in this population.
 
12:00    성감염증 관련 시장을 위한 Point of Care 검사 개발 실현
 
Joany Jackman, PhD, Investigator, Center for Point of Care Tests for STD, Johns Hopkins University School of Medicine*
 
The mission of the Johns Hopkins University Center for Point of Care Tests for Sexually Transmitted Diseases (JHUC) is to provide expertise, guidance and samples to enable the development of the best available test platforms for diagnosis of sexually transmitted infections (STIs). To that end JHUC has conducted focus groups, facilitated meetings and other studies to determine the most important attributes of successful test for STIs in a variety of point of care settings.  These data and their relevance to the global market for POCT for STIs will be presented.*In collaboration with: M.Jett-Goheen, A.Rompalo, T.Hogan, C.Gaydos
 
12:30    오전 세션 종료
 
생물학적 표적의 새로운 탐색 전략
 
2:00        직접 다중화 바이러스 탐색
 
John H. Connor, PhD, Professor, Dept of Microbiology, Boston University School of Medicine
 
We have developed an LED-based virus detection technology that is label-free and multiplexed. This technology allows the identification of viruses that cause hemorrhagic fever without the need for nucleotide isolation and amplification on a rapid time-scale in platform that can be used at the point of care.
 
2:30        유비저의 신속 진단을 위한 LFI(Lateral Flow Immunoassay)의 최적화
 
David AuCoin, PhD, Research Assistant Professor, Dept Microbiology and Immunology, University of Nevada School of Medicine
 
Burkholderia pseudomallei is a soil-dwelling bacterium that is the causative agent of melioidosis.  Laboratory detection of B. pseudomallei is difficult and slow, because of challenges with culturing and a lack of validated diagnostic reagents, but this has been the best approach for diagnosis of melioidosis.  Our goal, therefore, has been to develop a rapid point-of-care immunoassay for the diagnosis of melioidosis.  Our initial efforts have focused on developing a CPS-specific monoclonal antibody (mAb).  The same mAb was used to produce a prototype lateral flow immunoassay (LFI) that is capable of detecting CPS in a variety of patient samples. The CDC is currently testing the LFI against a large panel of B. pseudomallei, related and near neighbor strains.  Results of these tests and plans for further field testing will be reported.
 
3:00        PCR 프리, 시각적 DNA 탐색 기술 개발
 
Mahesh Uttamchandani, PhD, Assistant Professor, DSO National Laboratories, National University of Singapore, Singapore
 
Novel methods to detect DNA sequence specifically through color change reactions will be described. To transduce molecular recognition events into visual readouts, we have engineered assays which have exploited split DNAzymes and gold nanoparticles. The G-quadruplex DNAzymes were successfully applied to the detection of Salmonella and Mycobacterium DNA, as well as in genotyping a single base difference from within human genomic DNA samples.  An integrated workflow was capable of detecting DNA samples through a color change within just 5-15 minutes. The gold nanoparticle method offered much greater sensitivity, lowering the limit of detection visually, without the need for PCR amplification.
 
3:30        휴식시간, 전시회 관람
 
4:00        Point of Care 응용에 초점을 맞춘 전자유체역학 기술
 
Christian Reis, Group Lead - Biotechnology Processes, Fraunhofer IPA, Germany
 
Magnetic bead handling is a common tool in on-chip biodetection systems and research is improving fast.  A technology for detecting the load of a magnetic particle by forcing the particle to describe certain trajectories with switching magnets will be presented. This allows us to net-focus magnetic beads in a hydrodynamic system and to provide quantitative insight for the number of molecules bound to the particle surface.
 
4:30       클라우드 맵핑과 LIF 분류를 가능하게 하는 LIDAR 기술을 이용한 바이오에어로졸 스탠드 오프 탐색
 
Sylvie Buteau, PhD, Scientist, Defence Research and Development Canada, Canada*
 
A standoff sensor called BioSense was developed to demonstrate the capacity to map, track and classify bioaerosol clouds from a distant range and over wide area.  The concept of the system is based on a two steps dynamic surveillance: 1) cloud detection using an infrared (IR) scanning cloud mapper and 2) cloud classification based on a staring UV Laser Induced Fluorescence (LIF) interrogation. The main challenge is classification, which relies on a spectrally resolved UV LIF signature library. The system showed good performances even prior to further optimization. *In collaboration with: J.-R.Simard, G.Roy, P.Lahaie

5:00        iTIRF-분자 진단을 위한 휴대폰 기반 바이오센서
 
Alexander N. Asanov, PhD, President and CSO, TIRF Labs, Inc
 
A novel molecular diagnostics technology based on Total Internal Reflection Fluorescence, termed - iTIRF, will be presented.   iTIRF is capable of simultaneously detecting proteins, nucleic acids, and metabolite biomarkers.  iTIRF microarrays employ silk fibroin, which allows for much greater immobilization of reagents and a resulting signal that is a thousand-fold greater than that with classical TIRF.  Additional advantages of the biosensor, and plans for further development, will also be described.
 
5:30        병원균 추적과 식별을 위한 게놈 기반 어프로치
 
Willy Valdivia-Granda, PhD, CEO, Orion Integrated Biosciences Inc.
 
The microbiome of an animal contains approximately 10 times the number bacterial cells than host cells and around 150 times more genes.  Using a library of motif fingerprints and genomic signatures for pathogens of biodefense and agrodefense relevance, we performed an extensive survey of the metagenomic samples of humans and domestic animals.  We have used our motif fingerprint scanning technology to perform inclusion/exclusion bioforensic and attribution analysis.  The implications of our work in biosurveillance and standardized nucleic acid- or antibody-based detection system development will be discussed.
 
6:00        전시기업/스폰서 기업 프레젠테이션-I/최후 토론
 
6:15        전시회장 환영 리셉션과 포스터 발표 관람
 
 
 
2014년 6월 11일(수)
 
8:00        전시회/포스터 발표 관람, 커피와 다과
 
화학물질과 독소 탐색
 
9:00        환경 시료에서의 활성 리신 검사를 위한 신규 세포 기반 어세이
 
Baolin Zhang, PhD, Senior Investigator, Division of Therapeutic Proteins, Office of Biotechnology Products, Food and Drug Administration
 
Ricin is a deadly protein toxin with potential use as a bioterror agent. The threat of ricin attack has increased over the past decade and it has been linked to over a dozen criminal cases. The purpose of this project is to advance the current science of detection of active ricin based on its binding to the cell surface by functional ricin Chain B, cellular uptake of the catalytic Chain A, and subsequent cell death. The ability to discriminate between active and 'dead' ricin forms with the cell-based analysis can provide additional information on the risk factors associated with the sample.
 
9:30        광학 바이오센싱 시스템 기반 Microcystin-LR 자동 온라인 실시간 탐색
 
Han-Chang Shi, PhD, Professor, School of Environment, Tsinghua University, PR China
 
To minimize the health risks to the public, cyanotoxin detection methods that are rapid, sensitive, real time, and high frequency must be established.  An novel automated optical biosensing system (AOBS) was developed for the rapid detection of microcystin-LR (MC-LR). Results using an indirect competitive detection mode will be presented.   The quantification of MCLR ranges from 0.2 to 4 µg/L, with a detection limit determined as 0.09 µg/L.
 
10:00    Microcantileve가 실현되는 바이오탐색
 
Rick Venedam, PhD, Senior Scientist, National Security Technologies, LLC
 
Embedded piezoresistive microcantilever (EPM) sensors have been used in the detection of a variety of analyte species.  EPM sensors utilize a tiny piezoresistive microcantilever partially embedded into a sensing material to produce a sensing element that is compact, simple, resistant to movement and shock, and suitable for remote sensing applications. In this project  we used sensing materials consisting of an immobilizing polymer functionalized with either target enzymes or antibodies to detect two biological agents, Bacillus subtilis and Diisopropyl fluorophosphates, a simulant for organophosphate nerve agents.  Sensing results are presented for both types of EPM sensors.
 
10:30    휴식시간, 전시회/포스터 발표 관람
 
11:00    포토닉 노즈:다용도의 단순한 탐색툴
 
Leonardo Bonifacio, PhD, Research Scientist, Opalux Inc., Canada
 
The Photonic Nose platform, which is the first platform to make use of photonic crystals as an artificial nose, will be presented. It is a simple, cost-effective and versatile chemical sensing platform applicable for the detection of various analytes both in gas and liquid phases.  Most current nose technology is based on relatively complex and costly platforms.  The photonic nose can be used for analysis of both liquid and vapour phase samples, and is based on arrays of specially designed photonic sensors.  The combinatorial response can be analyzed by use of simple digital cameras for remote and near instantaneous verification.  We have implemented proof-of-concept projects that addressed challenges in areas that include bacterial detection and identification, safety testing for food, beverages, water and crude oil quality, and a number of other applications.
 
11:30    MEMS 기반 새로운 마이크로 코리올리 농도 측정 기술
 
Mike Touzin, Endress+Hauser Flowtec, Switzerland
 
Laboratory and field hazardous area applications of a novel MEMS technology for density measurement will be presented.  This microfluidic sensor, based on the Coriolis principle, can measure density/specific gravity, temperature and viscosity.  These very compact MEMS devices are immune to vehicular vibration and have an extremely fast response time, due their high resonant frequencies.  The ability to differentiate between types of fuels such as gasoline, ethanol, methanol, diesel, biodiesel, butanol, and to detect water and air contamination using density measurement, will be demonstrated. Concentrations of fuel blends such as E85 and others can be accurately determined.
 
12:00    분자 인식을 위한 Immunosignatures QC(QC) 레이저
 
Mariano Troccoli, PhD, Director of Product Development, AdTech Optics, Inc.
 
Recent results with high performance mid-infrared quantum cascade lasers both for high power and single-mode operation will be presented.  In addition, their applications to molecular recognition will be described and results on multi-wavelength detection of important chemical compounds in a single multi-laser system are detailed.
 
12:30      오전 세션 종료
 
2:00        Immunosignatures에 의한 숙주 반응 기반 바이오탐색
 
Stephen Albert Johnston, PhD, Co-Director, Center for Innovations in Medicine, Professor, School of Life Sciences, Biodesign Institute, Arizona State University
 
Most of biodetection efforts have focused on sensing the pathogen. This has serious basic and practical limitations. A simple technology based on immunosignatues, for detecting host changes in response to pathogens, will be presented.  It is very sensitive and inexpensive.  It is commercializable and importantly would enable new levels of biosecurity as a by product of standard clinical practice.
 
탐색을 위한 툴 - 미세유체공학와 효소
 
2:30        개발 시간을 단축, 리스크를 감소하기 위해 개발된 미세유체공학 컴포넌트 툴박스
 
Leanna Levine, PhD, CEO, Aline, Inc.
 
A toolbox of engineered microfluidic components, including metering channels, valves, vents, pumps, and de-bubbling, can be engineered into any number of desired footprints.  Optimized actuation inputs and protocols, and design specifications ensure well characterized and repeatable performance.  Through choice of materials and design constraints, we demonstrate data on the repeatable performance of a device that meters, mixes, debubbles and dispenses. Data is presented on component reproducibility and scalable production.

3:00        SoundStream-휴대용 고속 진단 장비를 위한 미세유체공학 기반 어세이 플랫폼
 
Arlene Doria, PhD, CEO, DEFINEQA Inc.
 
An innovative microfluidic technology known as SoundStream, will be described.  The field of microfluidics is plagued with challenges in integration, fluid control, and limited sample preparation strategies.  SoundStream employs the use of oscillating microbubbles to perform multiple assay steps including pumping, mixing, bead assay detection, plasma/serum separation, cell lysis, and particle size separation.  The technology is easy to integrate with bioassay detection methods.  It reduces the complexity of the microchip design and is scalable. Finally, the platform can be powered by simple batteries for rapid portable diagnostics.
 
3:30        휴식시간, 전시회/포스터 발표 관람
 
4:00        원핵생물 DNA의 특이적 농축을 위한 재조합 토포이소머라아제 응용
 
Natalia Sandetskaya, PhD, Fraunhofer Institute for Cell Therapy and Immunology IZI, Germany
 
The development and application of the novel molecular tool for the targeted enrichment of prokaryotic DNA in complex samples will be presented.  The DNA binding subunit of the bacterial topoisomerase II, gyrase, was expressed, purified and immobilized on magnetic particles. Results showing specific affinity towards bacterial DNA in the samples with high background of eukaryotic DNA will be described. This method is a promising approach for the preparation of such type of the samples, for example, in molecular diagnostics of sepsis.
 
4:30        열안정성이 높은 신규 바이러스 DNA 폴리머라아제가 촉진하는 DNA 및 RNA 표적의 Point of Care 분자 탐색
 
Hemanth Shenoi, PhD, Lucigen Corp
 
Point of care (POC) molecular detection of pathogens requires improvements in enzymes, formulation and stability. OmniAmp enzyme is a novel isothermal amplification polymerase for loop-mediated amplification (LAMP) amplification of RNA or DNA.  The unique inherent reverse transcriptase activity of the enzyme allows single enzyme detection of RNA targets.  OmniAmp can be formulated dry for ambient storage and transport. Detection of amplification products can be accomplished using multiple methods.
 
5:00        전시기업/스폰서 기업 프레젠테이션 - II/우수 포스터 발표 소개
 
5:30        정리, 컨퍼런스 종료

* 주최측 사정에 따라 사전 예고없이 프로그램이 변경될 수 있습니다.

 

 
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