- 단세포 분석 -

단세포 분석을 테마로 한 이 심포지엄에서는 시장을 크게 변화시킬 가능성이 있는 새로운 단세포 분석 기술의 최신 정보가 소개되며, 이 기술의 이점을 기존 기술과 비교 대조하는 세션 등이 예정되어 있습니다.

Recommended All Access Package:

11월 27일: 단세포 분석

11월 27일 Dinner Course: 쇼트코스 3:장기 칩 시스템의 기점, 최적화, 응용

11월 28-29일: 암면역요법과 병용요법을 위한 전임상 모델

11월 29-30일: 중추신경계 모델과 중개연구 전략

11월 29일 Dinner Course: 쇼트코스 4:Drug Discovery 연구자를 위한 면역학 기초 강좌, 파트 2:암면역과 자가면역

11월 27일(화)

7:00 Registration and Morning Coffee

전사체학(TRANSCRIPTOMICS) 기반 방법

8:20 Welcome Remarks

Joel Hornby, BSc, Conference Director, Cambridge Healthtech Institute

8:25 Chairperson’s Opening Remarks

Dimitry Ofengeim, PhD, Lab Head, Neuroimmunology, Neuroscience, Sanofi

8:30 Therapeutic Approaches to the Nervous System: One Cell at a Time

Dimitry Ofengeim, PhD, Lab Head, Neuroimmunology, Neuroscience, Sanofi

Single-cell RNA sequencing (scRNA-seq) is a sequencing platform that enables the analysis of transcriptomes from individual cells and is ideally suited to address the inherent complexity and dynamics of the central nervous system. There has been recent progress and challenges in applying scRNA-seq to advance our understanding of the brain in the application of this technology in the discovery of therapeutic targets and biomarkers for neurodegenerative diseases.

9:00 Single Cell Transcriptomic Analysis Workflow for Characterizing Complex Datasets of a Novel Human Corticogenesis Model

Marilisa Neri, PhD, Data Scientist, NIBR, Novartis

Single cell RNAseq is used to characterize a novel in vitro model of corticogenesis. Using our internally developed workflow, the neuronal differentiation has been characterized at cell granularity level: cell populations, marker genes and phenotype heterogeneity. Novel developed method for rare/sub cell types identification, CellSIUS (Cell Subtype Identification from Upregulated gene Sets), enabled characterization of a novel protocol that recapitulates the full spectrum of cortical development, including upper-layer corticogenesis in vitro.

9:30 Single Cell Transcriptomics of Patient Specific iPSC-Derived Motor Neuron Cultures for ALS Disease Modeling

Shila Mekhoubad, Scientist II, Stem Cell Biology, Biogen

10:00 Coffee Break

멀티 오믹스 분석법

10:30 The Impact of Cell Proliferation and Microenvironment on Tumour Heterogeneity

Anders Ståhlberg, PhD, Associate Professor, Clinical Pathology and Genetics & Sahlgrenska Cancer Center, University of Gothenburg & Sahlgrenska University Hospital

Here, we will show how cell proliferation and cancer stem cell properties contribute to intratumor heterogeneity in two entities: breast cancer and liposarcoma. We will also describe how the microenvironment determines the cellular phenotype of individual cells. To outline cell fate mechanisms we employed various single-cell techniques and functional assays.

11:00 Subcellular Spatial Analysis of Transcriptome and Proteome during Early Development

Radek Sindelka, PhD, Senior Scientist, Department of Gene Expression, Institute of Biotechnology, Czech Academy of Sciences, BIOCEV

Starting from a single fertilized oocyte, through manifold of divisions a complex organism is developed that has distinct asymmetries. One of the main challenges in developmental biology is to understand how and when these asymmetries are generated and how they are controlled. The African clawed frog (Xenopus laevis) is an ideal model for studies of early development thanks to their very large oocytes. We have developed a unique platform based on RT-qPCR, RNA-Seq and UPLC-ESI-MS/MS to measure asymmetric localization of fate determining RNAs and proteins within the egg and among the early stage blastomeres.

11:30 Sponsored Presentation (Opportunity Available)

12:00 Luncheon Presentation (Sponsorship Opportunity Available) or Enjoy Lunch on Your Own

12:30 Session Break

새로운 단세포 분석법

13:25 Chairperson’s Remarks

Dimitry Ofengeim, PhD, Lab Head, Neuroimmunology, Neuroscience, Sanofi

13:30 The Future of Pre-Implantation Genetic Testing - Accurate and Non-Invasive

Xiaoliang Sunney Xie, PhD, Lee Shau-kee Professor and Director of the Beijing Innovation Center for Genomics at Peking University, and Harvard University Visiting Professor, Biodynamic Optical Imaging Center, Peking University

Single-cell whole-genome amplification is critical for IVF. Current whole-genome amplification (WGA) methods present low accuracy of copy-number variation (CNV) and low amplification fidelity. We developed MALBAC and LIANTI, achieving the highest precision for single-cell genome sequencing with quasi linear and linear amplification, respectively, as opposed to exponential amplification in PCR. Based on these, we developed MARSALA, NICS and MaReCs, to successfully select fertilized eggs free of chromosome abnormalities and devastating point mutations in more than 300 couples.

14:00 Metabolic Imaging of Single Cells Using Mass Spectrometry

Ian Gilmore, Senior NPL Fellow, National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory (NPL)

14:30 Single Cell Mass Spectrometry in the Context of Drug Discovery

Carla Newman, Investigator, Ex vivo Bioimaging, GlaxoSmithKline

A paradigm shift in the drug discovery workflow is required to reduce attrition and transform conventional drug screening assays into translatable analytical techniques for the analysis of drugs in complex environments, both in vitro and ex vivo. We propose the use of two mass spectrometry techniques: SIMS and static electrospray mass spectrometry to visualise unlabelled compounds inside the cell at physiological dosages that can offer valuable insight into the compound behaviour both on and off-target.

15:00 Refreshment Break

15:30 Single Cell Antibiograms

Piotr Garstecki, PhD, Professor, Microfluidics and Complex Fluids, Institute of Physical Chemistry, Polish Academy of Sciences

Droplet microfluidics is an ideally suited technology for digital assays for bacterial cell counting and offers the possibility to probe the response of bacteria to antibiotics at the single cell level. The technology offers several interesting features – including alleviation of the unwanted inoculum effect in antibiogram assays and information on the distribution of susceptibility in the population.

16:00 Decoding Neuronal Diversity by Single Cell Convert-Seq

Joachim Luginbuehl, PhD, JSPS Fellow, RIKEN Yokohama

We present Convert-seq, combining single-cell RNA sequencing (scRNA-seq) and pooled (mutiplexed) ectopic gene expression with a new strategy to discriminate sequencing reads derived from exogenous and endogenous transcripts. We demonstrate Convert-seq by associating hundreds of single cells at multiple time-points during direct conversion of human fibroblasts to induced neurons (iN) with exogenous and endogenous transcriptional signatures. Convert-seq is a broadly applicable workflow to rapidly identify key transcription factors orchestrating the direct conversion of virtually any cell type.

16:30 Droplet Microfluidics for High Throughput Biopharmaceuticals Screening

Håkan Jönsson, PhD, Assistant Professor, KTH Royal Institute of Technology

Biopharmaceuticals make up the majority of highest grossing drugs. Many protein therapeutics are produced in mammalian cell factories such as CHO or HEK293. Droplet microfluidics allows for single cell level high-throughput analysis of protein secretion. We present a platform based on split-GFP complementation in droplets, which enables screening of a heterogeneous variant library, transfectant pool enrichment by protein secretion and selection of a highly productive clone.

17:00 Close of Symposium


18:0020:30 Recommended Dinner Short Course*

쇼트코스 3:장기 칩 시스템의 기점, 최적화, 응용

* Separate registration required.

* 주최측 사정에 따라 사전 예고없이 프로그램이 변경될 수 있습니다.

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