7th International Conference Sample Prep 2013 - 제7회 국제 시료 조제 컨퍼런스, 2013 -
2013년 5월 9 - 10일, 미국 캘리포니아주 샌디에고 San Diego Hilton & Resort

 
         
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SAMPLE PREP 2013은 Knowledge Foundation이 각지에서 개최하고 있는 Sample Prep 컨퍼런스 시리즈 중 하나로, 시료 조제 기술 전문가를 대상으로 국제적으로 높은 평가를 받고 있는 컨퍼런스입니다. 생물의학, 생물학, 화학 분야의 약제와 물질, 위협을 Point-of-care, 연구실, 각종 현장 환경에서 검출, 분류, 진단, 분석하기 위한 시료 조제 기술에 초점을 맞춘 컨퍼런스로, 최신 연구개발 성과, 시장 투입 예정 기술, 각종 용도 등이 소개됩니다. 올해 컨퍼런스에서는 다음과 같은 토픽이 다루어질 예정입니다.

- 시료 수집, (사전) 농축, 용해, 표적 추출을 위한 확실한 방법

- 조기진단에서 시료 조제의 중요한 역할

- 신속한 샘플-투-시퀀스(sample-to-sequence) 기술

- Point-of-care 샘플링, 검출, 분석

- 미세유체공학(microfluidics)을 이용한 시료 조제

- on-a-chip 시료 조제

- 로버스트 샘플링법(시료 조제의 자동, high-throughput, combinatorial approach)

- 고감도 시료 조제를 위한 각종 증폭법

- 분리 시스템 및 통합 모듈 어프로치에 의한 시료 조제

- 바이오탐색(biodetection)과 샘플링 기술 및 디바이스를 도입할 가능성이 높은 최종사용자

- 시료 조제와 각종 응용 기술에 대한 대체 어프로치와 혁신적 어프로치

- 현장에서 사용가능한 기기 - 호환성/신뢰성/확장성

 

이 컨퍼런스에서는 생물방어(biodefense), 현장 및 Point-of-care에서의 생물의학적 응용과 임상 응용, 식품 및 물 검사, 환경 및 농업 분야에서의 샘플링 등에 이용가능한 새로운 로버스트 샘플링(robust sampling)과 생물법의학 기술도 소개될 예정입니다. 또한 정부 기관과 대학, 기업 등에 소속된 정상급 전문가들이 등장하여 기술개발과 실용화의 초점이 되고 있는 다음과 같은 이슈와 영역에 대해 논의합니다.

- 표적 강화와 백그라운드 디플레션(background depletion) 기술

- 세포 및 분자 수준에서 희박한 물체(dilute objects) 분리

- 비혼합상(Immiscible phase) 정제

- 질긴(여과 불가능) 기질로부터 추출을 가능하게 하는 새로운 방법

- 차세대 시퀀싱을 위한 핵산 및 단백질 기반 시료 조제

- 시료 조제를 위한 나노테크놀러지와 미세화 문제

- 시료 조제, 검출, 분석을 위한 새로운 어세이와 시퀀싱 기술

- 각종 용도에서의 시료 조제 표준화와 규제 문제

- 검출/진단 vs. 의약품을 위한 시료 조제 기술


 
 
 

미디어 스폰서 및 컨퍼런스 파트너



 



 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 
 
2013년 5월 9일(목)
 
8:00        등록, 전시회 관람/포스터 발표 준비, 커피
 
8:50        주최측 환영사 및 개회사
 
9:00        차세대 바이오센서 개발이 각종 시료 조제 어프로치에 미치는 영향
Charles Young, PhD, Principle Professional Staff, Asymetric Operations Dept, The Johns Hopkins University Applied Physics Laboratory; Assistant Research Professor, Dept of Geography and Environmental Engineering, The Johns Hopkins University
Biological sensors represent one facet of a system designed to identify a biological agent. In addition to the sensor, a detection system must account for sample acquisition, sample storage/transport, sample preparation, assay design/performance and data management. Therefore, a systems approach is critical for the development of next generation sensors since changes to one component can impact the entire system. Over the past 5 years, researchers at JHU/APL have been tracking biosensor development and building a database of commercial-off-the-shelf systems and their capabilities. In this presentation we will discuss the database, current sensor trends and their implication for sample preparation.
 
9:30        Point of Need 진단을 위한 시료 조제 어프로치
Richard Allen, PhD, Senior Scientist, Biodefense and Food Safety, Luminex Corporation
Complex biological states such as transmissible infectious disease involve a dynamic relationship between pathogenic organisms and their hosts. Accurate assessment of this relationship presents a unique instrumentation and assay challenge. To provide a more comprehensive snapshot of an evolving infection, efforts at Luminex Corporation include development of an automated, field portable diagnostic system based on the open architecture xMAP technology. A key aspect of this system is the ability to test for both the pathogen itself, through molecular techniques, and also host biomarkers that signal the course of infection via affinity capture reagents. Taken together, these data will yield a more accurate and timely treatment, leading to better clinical outcomes than either test alone. The challenge in developing such a system is to devise sample preparation protocols that 1) are versatile enough to encompass either technique, 2) can be deployed onto an automated, inexpensive disposable cartridge, and 3) are rapid enough to both inform clinical decisions and provide multiple measurements throughout disease progression. Luminex will provide an update on sample preparation techniques being investigated for this effort and provide data from initial testing.
 
10:00    시료 배송 시스템 컨셉
John C. Carrano, PhD, President and CEO, Paratus Diagnostics, LLC
In this paper we posit that in order to realize pragmatic point-of-care medical diagnostic devices capable of meeting the rigorous requirements of CLIA waiver, that one must solve the inherent "impedance mismatch" between the clinical acquisition of a human patient sample and its delivery to the POC Dx system or device. We work from the assumption that specimen acquisition from the patient must follow standard clinical practice, and that the POC system (e.g. "device") will be designed to follow a yet to be determined international interface standard (similar in principle to the now ubiquitous Luer-Lock standard). We will present a design concept for a "specimen delivery system" and the associated proposed interface standard, along with experimental data from preliminary rapid prototype models.
 
10:30    휴식시간, 전시회/포스터 발표 관람
 
11:00    실시간 메타게노믹스(Metagenomics):산호초 연구에서 임상 응용으로의 이동
Forest L. Rohwer, PhD, Professor of Biology, San Diego State University
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
11:30    병원체 생사판정 PCR(중합효소 연쇄 반응) 검사를 위한 기술
Sarah Fakih, PhD, Senior Scientist, Food Safety Testing R&D, QIAGEN GmbH, Germany
Nucleic acid detection methods, such as real-time PCR, provide fast and powerful tools to analyze samples for the presence of potentially harmful microbes, but also hold the risk of false positives by detecting nucleic acid from harmless dead cells. We addressed this by a universally applicable tool box for an advanced viability PCR, which centers on the DNA-masking compound Propidium monoazide (PMA), suppressing amplification signals from dead cell DNA. We have pre-developed viability PCR as a complete standardized system to be used with a new illumination device designed to catalyze the PMA reaction. The new tool box system, the illumination device and its function together in viability PCR applications are presented with multiple sets of user data.
 
12:00    Point-of-care 환경을 위한 견고한 자족형(Self-Contained) 시료 조제 카트리지
Season Wong, PhD, Co-Founder and Director, AI Biosciences, Inc.
This presentation will cover the development of AI Biosciences Inc.'s Sample Preparation Cartridge (SPC) which delivers high quality nucleic acids for point-of-care molecular analysis. Our SPC can be operated manually or hands-free using battery pack. The SPC is a closed-system that eliminates cross-contamination and provides hassle-free waste disposal. We will demonstrate its utilities using a wide range of samples. The detection of the derived nucleic acid in a non-laboratory setting will also be discussed.
 
12:30    Knowledge Foundation 멤버십 프로그램 제공 오찬회
 
2:00        만성 림프구성 백혈병(CLL) 환자의 혈액으로부터 cfc-DNA의 분리와 검출 - '샘플-투-시퀀스(sample-to-sequence)의 신속화'
Michael J. Heller, PhD, Professor, Depts of Bioengineering and Nanoengineering, University of California San Diego*
We have developed a unique sample to answer dielectrophoretic (DEP) technology for rapid isolation and detection of cancer related cell free circulating DNA (cfc-DNA) biomarkers, bacteria and virus directly form blood and other complex biological samples. Using this DEP microarray device, cfc-DNA from chronic lymphocytic leukemia (CLL) patients could be isolated directly from 50uls of unprocessed whole blood in about 15 minutes. The cfc-DNA was then eluted from the DEP chip, PCR amplified using CLL specific primers and sequenced to determine CLL patient specific polymorphisms and mutations. Overall, the use of DEP devices for the rapid isolation of cfc-DNA from a small volume of blood will allow "noninvasive liquid biopsy" point of care (POC) detection and diagnosis of incipient, residual, and recurrent cancer and other diseases.
*In collaboration with: Jennifer Marciniak and Avery Sonnenberg
 
2:30        환경내 생물학 작용제를 검출하기 위한 일반적인 시료 조제 어프로치
Martin McDonnell, PhD, Principal Scientist, Detection Department, Defense Science and Technology Laboratory - DSTL Porton Down, United Kingdom
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
3:00        혈액으로부터 세균 시료를 신속히 조제하는 방법
Alexis Sauer-Budge, PhD, Senior Research Scientist, Fraunhofer Center for Manufacturing Innovation, Fraunhofer USA
Traditionally, bacterial pathogens in the blood have been identified using culture-based methods that can take several days to obtain results. This can lead to physicians making treatment decisions based on an incomplete diagnosis contributing to patient morbidity. To decrease diagnosis time, we are developing a novel sample preparation device for isolating and concentrating dilute bacteria from blood. The device is designed to be a single-use disposable that can be used manually or with a fully automated instrument.
 
3:30        휴식시간, 전시회/포스터 발표 관람
 
4:00        라만분광법에 의해 물의 미생물 오염을 시약없이 신속히 분류하는 방법
Gregory Auner, PhD, Professor, Electrical and Computer Engineering, SSIM Director, Wayne State University
We are developing a reliable and rapid method to assess microbial contamination in water. Raman Spectroscopy is a reagentless, non-destructive, technique that provides a unique spectral fingerprint of bacteria without sample preparation and is conducive to field application since it can provide both qualitative and quantitative analysis. We will present Raman identification of E. coli, Salmonella, Rahnella aquatilis, Enterobacter, Vibrio fluvialis, Pseudomonas, Bacillus subtilis, Listeria, Staphylococcus, and Streptococcus.
 
4:30        덴드리머(Dendrimers)에 의해 수정된 다중벽 카본나노튜브(MWCNTs)를 기반으로 한 인간 세포 프리온의 Label-Free Electrochemical Aptasensor
Hafsa Korri-Youssoufi, PhD, Researcher, Institut de Chimie Moléculaire et des Matériaux d’Orsay - ICMMO, University Paris-Sud, France
The present work aims to develop an electrochemical aptamer based biosensor able to detect human cellular prions PrPC as a model biomarker of prion disease with high sensitivity. We designed biosensor using multiwalled carbon nanotubes (MWCNTs) modified with polyamidoamine dendrimers (PAMAM) of fourth generation (G4) able by their amino group to attach ferrocenyl redox marker and the DNA aptamer as bioreceptor. MWCNTs, thanks to their nanostructure organization and electrical properties, allow the distribution of aptamer and redox markers over the electrode surface. We demonstrated that the interaction between aptamer and prion protein leads to variation in electrochemical signal of the ferrocenyl group. High sensitivity detection limit of 0.5 pM and wide linear range of detection from 1 pM to 10 µM has been demonstrated. Detection of PrPC in spiked blood plasma has been achieved and demonstrated a recovery of 85%.
 
5:00        카트리지형 Point-of-care HIV 바이러스량 검사용 노스핀(No-Spin) 시료 조제 기술
Shan Gao, PhD, Senior Chemical Engineer, Wave 80 Biosciences, Inc.*
Porous polymer monoliths (PPMs) were developed to prepare nucleic acids from HIV virion-spiked whole blood or plasma samples for quantification of viral RNA using both standard qRT-PCR assays and a novel high-sensitivity bipartite signal amplification assay (BSAA). The efficiency of optimized in-cartridge, glycogen-mediated and monolith-based extraction of viral RNA was approximately 50%. This is comparable to standard centrifuge-based methods involving either phenol-chloroform extraction s or commercial spin columns. The new sample preparation method is particularly well suited for the development of point-of-care HIV viral load testing systems with sophisticated functionality and low cost.
*In collaboration with: F.Wang, H.Negussie, J.Frey, A.Arsham, A.Droitcour, L.Mazzola, D.Laser, Wave 80 Biosciences; and A.Fan, C.Klapperich, Boston University
 
5:30        전시기업/스폰서 프레젠테이션
 
6:00        첫째날 종료
 
 
 
2013년 5월 10일(금)
 
8:00        전시회/포스터 발표 관람, 커피
 
9:00        Fluid-on-Board
David Wright, CEO, Wi Medical Device Development, Inc.
This talk is aimed at the methods of designing a fully integrated self-contained IVD cartridge. The evolving world of complex IVD test cartridges is moving towards a self-contained format, where the fluidic assay is contained, from sample pre to waist containment. The need for this format is based on taking complex assays from the lab into the field, where persons of moderate skills may execute a test with confidence, in a short period of time, at acceptable cost. The world of diagnostics is changing the world of applied therapy, and thusly, the cost of healthcare.
 
9:30        임상 시료로부터 라임병 보렐리아 부르그도르페리(Borrelia burgdorferi)를 직접 검출하기 위한 개량된 시료 조제 및 표적 강화 기술
Mark W. Eshoo, PhD, Director of New Technology Development, Ibis Biosciences Inc., an Abbott Company
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
10:00    MEMS-미세유체공학의 통합 기준과 설계:자기 비드(Magnetic Bead) 시료 농축/검출 기술 사례
Mark Tondra, PhD, President, Diagnostic Biosensors
Commercialization of Point of Care (POC) and integrated Lab on a Chip (LOC) products is advancing. One enabling factor is industry standards for microfluidic interfaces between two devices, like a detector chip and a concentrator chip. These standards facilitate the design of higher-level micro-scale systems using plug-and-play architectures. The state-of the art in MEMS-Microfluidics industry standards will be presented. Our own use of these standards in design of multi-tube fluidics interfaces in our biosensor products will be shown, as will applications of devices using magnetic microbeads for sample concentration and manipulation in microfluidic LOC systems.
 
10:30    휴식시간, 전시회/포스터 발표 관람
 
11:00    LC-ESI MS/MS 분석법에 따른 신속한 병원체 분류와 내성 예측을 위한 완전 자동화된 시료 조제
Patrick Broyer, PhD, Senior Scientist, bioMérieux SA, France
This talk will present a new automated sample prep solution for extraction of proteins and peptides dedicated to LC-ESI-MS analysis. Protocol simplifications, new filtration device for urine and blood & automation of peptide digestion will be described to show the ability of this solution to move to a long 18h protocol to a short 1h fully automated protocol. Results will be showed demonstrating the capabilities to correctly identify resistant microorganisms from positive blood culture. The sample preparation automated prototype is already used in our laboratory providing within 1 hour peptides solution ready to be analyzed by LC-ESI-MS by batches of 30 samples per run.
 
11:30    복잡한 환경 매트릭스로부터 생물학적 입자의 분별과 농축을 위한 새로운 막기반 시스템 개발
Andy Page, President & CTO, InnovaPrep LLC
InnovaPrep LLC will present an update on development of a novel, integratable, membrane-based fractionation and concentration system, under a current Department of Homeland Security Phase II SBIR. Powerful, automated sample preparation techniques are needed for improved detection and identification of rare biological particles in complex environmental samples by autonomous biodetection systems. A novel cartridge containing a series of sharp-cut membrane filters in order of decreasing pore size, along with integrated pneumatic valves and fluid paths, are used to rapidly separate particles present in up to 20 mL of environmental sample by size into separate fractions containing large environmental particles, bacteria, viruses and DNA, and proteins. Each captured fraction is then eluted into separate concentrated volumes of less than 200 µL. Program goals, hurdles, and performance data will be presented.
 
12:00    차세대 시퀀싱을 위한 자동화된 시료 조제 및 분석 기술
Numrin Thaitrong, PhD, Researcher, National Center for Genetic Engineering and Biotechnology / Sandia National Labs
Thorough characterization of next-generation sequencing library is a required process in the sample preparation workflow. However, this built-in quality control feature is currently not available for small-scale library construction platforms. We have developed an integrated droplet-based digital microfluidics (DMF) architecture with capillary interface to handle sub-microliter sample processing and analysis. The platform providing size distribution and quantization of the library can be coupled to the existing modular DMF "hub" to automate start-to-finish library construction.
 
12:30    중식
 
2:00        임상 시료 수집, 반송, 조제의 개선
Marek Smieja, MD, PhD, Associate Professor, Dept of Pathology and Molecular Medicine, McMaster University, Canada*
The development of point of care diagnostic tests for common infectious diseases needs parallel improvements in the ease of collection of clinical samples, and appropriate transport media for in-tube sample stabilization and/or extraction. We studied the effectiveness and reproducibility of Copan's FLOQSwabs™ for sample collection for respiratory, gastrointestinal, and genitourinary diseases. We determined the feasibility and performance characteristics of self-collected, less-invasive diagnostic samples; evaluated the robustness of transport media (dry swabs, UTM, eNAT, ESwab, MSwab, Cymol) under differing temperature and time conditions; and determined the performance characteristics of various media for antigen-based and molecular diagnostic tests.
*In collaboration with: Copan Diagnostics
 
2:30 Integrated PureLyse® Sample Preparation
Bruce Irvine, CTO, Claremont BioSolutions LLC
Claremont BioSolutions has created a novel method of rapid sample preparation that is entirely disposable. It provides mechanical cell lysis and simultaneously extracts nucleic acids or proteins. Nucleic acid can be extracted in three to five minutes with two to three steps. The disposable PureLyse® device is comprised of a micro-motor and vane that agitate particles at unusually high shear forces. As cells are lysed, nucleic acid binds to the lysing particles. This system delivers high yield RNA preparation as well. This system is also used to very effectively homogenize tissue. The miniature disposable nature of this flow-through cartridge lends itself to integration. Our flagship approach to integration is the OmniValveâ„¢ fluidic system, where we have embedded the PureLyse® chamber within a valve with up to six ports connecting the lysis and extraction cartridge to other chambers. This facilitates integration of DNA extraction with sample introduction, pre-filters, wash, elution, and amplification. This approach was very successfully applied to an NIH funded SBIR project developing a semi-integrated system for detecting Clostridium difficile, resulting in 100 % specificity and 96% sensitivity as compared to standard enzyme immunoassay of stool samples.

 
3:00        전혈에서 안정적인 유전자 발현:인간 혈액 시료의 상온 처리에 의한 고품질 RNA 정제
Vasco Liberal, PhD, Scientist, Biomatrica, Inc.
Expression profiles from blood samples are increasingly used to diagnose, monitor and treat diseases, requiring reliable RNA preservation during sample collection, transport and storage. Transcription profiles can change rapidly, potentially dictating inadequate treatment. Biomatrica has developed a blood collection device that stabilizes RNA in blood cells during ambient temperature sample handling and storage. With its coupled RNA purification kit, it provides a complete solution from blood collection to RNA purification, achieving high yields and great RNA quality, with unaltered gene expression for 14 days of room temperature storage, which can prove of extreme value for better patient treatment.
 
3:30        전시기업과 스폰서 프레젠테이션/우수 포스터 발표 소개
 
4:00        폐회사, 컨퍼런스 종료

 


컨퍼런스에서는 기업과 정부기관, 대학 소속 연구자의 포스터 발표가 진행됩니다. 포스터 발표를 희망하시는 분은 컨퍼런스 자료에 게재할 1 페이지 분량의 초록(8.5인치x 11인치, 여백 1인치)을 2013년 4월 5일까지 이메일로 보내주시기 바랍니다. 포스터 발표 응모는 4월 20일까지 접수하며, 경우에 따라 초록의 컨퍼런스 자료 게재가 불허될 수 있습니다.

포스터 보드 사이즈:
폭 4피트 x 높이 3피트
(포스터 보드는 세로형도 가능하며 이 경우, 폭 3피트 x 높이 4피트 : 90cm x 120cm)

주의: 포스터 발표에 응모하기 위해서는 컨퍼런스에 참가등록하셔야 하며, 사전에 등록비 및 포스터 보드 예약비를 결제하셔야 포스터 보드 게시 공간 예약이 완료됩니다.
 
전시회 전시

이 컨퍼런스에서는 전시와 스폰서에 관한 다양한 옵션이 마련되어 있습니다. 특히 호평을 얻고 있는 옵션은 아래와 같습니다. 자세한 사항은 웹페이지 상단 전시 안내 PDF 링크를 통해 확인하시거나 문의해 주세요.

10x10피트 전시 부스 비용:2,499달러

패키지 내용:
  • 전체 세션룸에 인접한 10 x10 피트 부스
  • 풀 컨퍼런스 패스 1매
  • 전시회장에만 입장가능한 부스 담당 직원용 패스 1매(패스 추가시 1매에 149달러)
  • 컨퍼런스 자료 1부
  • 컨퍼런스 자료 바인더에 8.5 x 11 인치 흑백 광고 삽입(199 달러의 추가요금으로 컬러 광고로 변경 가능)
  • 1회만 이용가능한 컨퍼런스 참가자 메일링 라벨 1부를 컨퍼런스 종료 후 송부
  • 귀사 고객 및 유망 고객에게 공동 다이렉트 메일
  • 컨퍼런스 웹사이트 전시기업 섹션에 전시기업 리스트와 로고 게재(귀사 웹사이트 상호 링크 포함)
  • 메인 프로그램의 'Product Showcase'에서 15분간 프레젠테이션
  • 컨퍼런스 전시 제품 및 서비스에 대한 자료를 게재한 귀사 웹사이트 링크를 컨퍼런스 e-newsletter에 게재
  • 메인 컨퍼런스 브로셔에 전시기업으로 게재(기한내 신청한 전시기업만)


Discount Accommodations and Travel:
A block of rooms has been allocated at the conference hotel. Please make your reservations by April 3, 2013 to obtain this rate. When making reservations, please refer to the Knowledge Foundation. Contact The Knowledge Foundation if you require assistance.

CONFERENCE VENUE:
San Diego Hilton & Resort
1775 East Mission Bay Drive
San Diego, CA  92109




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